针刺丰隆单穴及其配穴调节高脂血症大鼠血脂的效应比较

Efficacy comparison of needling Fenglong (ST40) and coordinated acupoints for regulating blood lipid level in rats with hyperlipemia

目的比较针刺丰隆单穴和其配穴调节高脂血症大鼠血脂的效应差异。方法采用高脂饮食饲料喂养制备高脂血症大鼠模型。将40只SD大鼠随机分为空白组、模型组、丰隆单穴组和丰隆配穴组(丰隆、关元、内关),每组10只。空白组每天给予基础饲料喂养,其余三组每天给予高脂饲料喂养。喂养2周后断尾取血测血脂。之后,给予丰隆单穴组、丰隆配穴组针刺治疗5周。治疗结束后,所有大鼠腹腔静脉取血检测血脂。结果丰隆单穴组及配穴组治疗前后的总胆固醇(TC)降低差值均较模型组有显著性差异(P<0.05)。丰隆配穴组治疗前后的低密度脂蛋白(LDL)降低差值、及高密度脂蛋白(HDL)升高差值均较模型组和丰隆单穴组有显著性差异(P<0.05)。结论针刺丰隆单穴和其配穴(丰隆、关元、内关)均可明显降低高脂血症大鼠的TC水平。但针刺丰隆配穴在降低高脂血症大鼠的LDL水平、及升高HDL水平方面,较针刺丰隆单穴有明显优势,说明针刺丰隆、内关和关元三穴在调节血脂方面有很好的协同作用。

Objective To compare the efficacy difference of needling in Fenglong （ST40） and the coordinated acupoints for regulating blood lipid level in rats with hyperlipemia. Methods The model was established by feeding high fat diet to SD rats and then the rats （n = 40） were randomly divided into the blank group, model group, ST40 group and coordinated acupoints group [ needling ST40, Guanyuan （RN4） and Neiguan （PC6） , each n = 10 ]. The blank group was fed basic diet everyday, while the other groups were fed high fat diet everyday. After 2 weeks the blood samples were taken from rat cut-off tails for testing blood lipid level. The acupuncture therapies, whereafter, were given to the ST40 group and coordinated acupoints group for 5 weeks. Finally the blood samples were taken from celiac vein in all rats for testing blood lipid level. Results The descend of TC differential values in the ST40 group and coordinated acupoints group had significantly difference compared with that of the model group （P 〈 0. 05 ） before and after the treatment. The descend of LDL differential value and ascend of HDL differential value in the coordinated acupoints group had significantly differences compared with those of the model group and ST40 group （ P 〈 0.05 ）. Conclusion Acupuncture in ST40 and the coordinated acupoints （ST40, RN4 and PC6 ） can obviously decrease TC level in rats with hyperlipemia, while acupuncture in the coordinated acupoints has better effects for decreasing LDL level and increasing HDL levels. The results show that the acupuncture in ST40, RN4 and PC6 has a coordinative effect on the regulation of blood lipid level.