HPLC法测定注射用硫普罗宁含量

Determination of Tiopronine Injection by HPLC

目的：建立用高效液相色谱法测定注射用硫普罗宁含量的方法。方法：采用WatersC18（4.6mm×150mm，5μm）色谱柱；流动相为乙腈．水（10：90），用磷酸调节pH值至2．5；检测波长为210nm；流速：1、0mL·min^-1。结果：硫普罗宁的浓度与色谱峰面积在10—300mg·L-1范围内成良好线性关系，r=0．9998，回收率99．66％，RSD为0.54％。结论：该方法操作简便、灵敏、准确、快速，专属性强，适用于测定注射用硫普罗宁的含量。

Objective：In order to establish the method of content determination of Tiopronine for Injection by HPLC. Method：Waters C18 （4. 6 mm × 150 mm,5μm） column was used, the mobile phase was composed of acetonitrile-water （ 10： 90）, adjusted pH to 2.5 by phosphoric acid, the detection wavelength was set at 210 nm. Flow rate：1. 0 mL · min^-1. Results：The linear range of Tiopronine was 10 - 300 mg· L^- 1. The recovery was 99. 66% with RSD 0. 54%. Conclusions： The new analysis method is simple, sensitive, accurate, rapid and specific. The method established in this paper can be adopted for the content determination of Tiopronine for Injection.