三七活性成分抗肿瘤作用及其免疫学机制初探

Antitumor effect and immune mechanism of active compositions from Panax notoginseng

目的通过测定小鼠黑色素瘤(B16)荷瘤小鼠外周血中肿瘤坏死因子(TNF-α)、白细胞介素-2(IL-2)的活性,探讨三七提取活性成分人参皂甙-Rh2(Rh2)、人参皂甙-Rg3(Rg3)抗肿瘤作用及对免疫功能的调节效应。方法将B16肿瘤移植后的小鼠随机分为Rh2大、中、小剂量组,Rh2+Rg3组,Rg3大、中、小剂量组,环磷酰胺(CTX)组以及模型组9组。计算肿瘤抑制率、胸腺指数及脾指数;放免法测定小鼠血清TNF-α、IL-2含量。结果Rh2与Rg3各剂量组均有一定的抑瘤效果,Rh2大剂量组、Rh2中剂量组、Rg3大剂量组以及Rh2+Rg3组与模型组瘤重比较,有统计学意义(P<0.05),其肿瘤抑制率分别为35.5%、30.1%、33.6%与38.6%。与CTX组比较,Rh2、Rg3单用及联合用药均能明显提高外周血中TNF-α、IL-2的含量,以联合用药组最为显著,且能够提高荷瘤小鼠免疫器官重量。结论三七提取活性成分Rh2、Rg3有一定的抗B16、提高免疫功能作用。而且联合用药具有协同增效作用。

Objective To investigate the antitumor effect and immunological regulation of ginsenoside Rh2 and ginsenoside Rg3 from Panax notoginseng through detecting the activities of tumor necrotic factor-or （TNF-α） and interleukin-2 （IL-2） in peripheral blood of mice with melanoma-B16. Methods After melanoma-B16 was transplanted the mice were randomly divided into 9 groups, including the Rh2 groups and Rg3 groups with different doses of ginsenoside Rh2 and ginsenoside Rg3 respectively, group Combined Rh2 and Rg3, CTX group and model group. The tumor-inhibitory rate, thymus and spleen indexes were calculated. The contents of serum TNF-α and IL-2 were detected by radioimmunoassay. Results There were some tumor-inhibitory effects in the Rh2 groups and Rg3 groups. The tumor-inhibitory rate in the high-dose and medium-dose Rh2 groups, high-dose Rg3 group and group combined Rh2 and Rg3 was respectively 35.5 %, 30.1%, 33.6 % and 38.6 %. It was significant statistically （P〈0.05）in tumor weight in above groups compared with that of the model group. Comparing to the CTX group ginsenoside Rh2 or ginsenoside Rg3 used separately or jointly, especially combined administration, all increased the contents of serum TNF-α and IL-2 as well as immune organ weight of mice with B16. Conclusion Ginsenoside Rh2 and ginsenoside Rg3 from Panax notoginseng has effects of inhibiting melanoma-B16, and improving immune function in mice. The combined administration of ginsenoside Rh2 and ginsenoside Rg3 is more efficacious.