应用微量稀释法对伊曲康唑于皮肤癣菌MIC影响因素分析

Analysis of the Factors of Influence on MIC of Itraconazole to Dermatophytes with Microdilution Method.

测定抗真菌药物 MIC的方法和程序急待标准化 ,多种实验因素可影响 MIC值。我们用微量稀释法测定了 2 0株红色毛癣菌对伊曲康唑的敏感性 ,用正交设计表 L9( 34 )法分析比较了不同 p H值、培养温度、接种密度及结果判读时间等对 MIC值的影响。结果显示在 RPMI 1640液基上 ,p H7.0、2 7℃培养、1× 10 5个孢子 /ml接种量以及 8天培养期为适宜实验条件。这些为今后测定丝状真菌对抗真菌药物敏感性实验的标准化打下了基础。

It is critical that the methods and procedures of determining the MIC of antifungal drugs are stan- dardized.Many testing factors can influence MIC values.Recently,we determined the susceptibility of 2 0 T.rubrum isolates to Itraconazole with microdilution method.Different p H values,incubation tem- perature,inoculum concentration and incubation time for MIC values were compared and analysed with the test of cross design table.The rdsults showed thatthe suitable testing conditions were p H7.0 ,incu- bation temperature2 7℃ ,inoculum concentration1× 10 5cells/ ml and incubation time8days on1640 liquid culture.This is a basis for the standardization of determining the susceptibility of antifungal a- gents to filamentous fungi in future.