DNA倍体分析和CEA在胸腹水鉴别诊断中的价值

The Value of DNA Ploidy Analysis and CEA in Differentiating Malignant from Benign Pleuroperitoneal Fluids

目的应用FCM-DNA倍体分析和CEA测定来探讨其对良恶性胸腹水的鉴别诊断价值。方法选取胸腹水120例,每位患者除常规胸腹水细胞学检查外,还进行FCM-DNA倍体分析和CEA检测。结果FCM-DNA倍体分析和CEA水平在良恶性胸腹水之间差异显著。与细胞学比较,DNA倍体分析敏感性最高83.6%,而细胞学的特异性最高100%,DNA倍体分析准确性最高89.2%。采用细胞学联合DNA倍体分析检测敏感性提高至91.8%,准确性高达95.8%。结论DNA异倍体和CEA水平异常是恶性胸腹水的特征,可作为细胞学检查的重要辅助手段,而细胞学联合FCM-DNA倍体分析可提高胸腹水细胞学的诊断准确率。

Objective To explore the diagnostic value of FCM-DNA ploidy, the test of CEA in differentiating malignant from benign pleuroperitoneal fluids. Methods 120 patients with pleuroperitoneal fluids were selected. Besides routine cytology examination, FCM-DNA ploid） analysis and CEA were detected. Results There were significant differences in FCM-DNA ploidy and CEA expression between malignanl and benign pleuroperitoneal fluids. Compared with cytology, the sensitivity of FCM-DNA ploidy was the highest（83.6%）, the specificity ot cytology was the highest（10%）, and the accuracy of FCM-DNA ploidy was the highest（89.2%）. Combined detection of DNA content ant CEA achieved 95.8% sensitivity and 91.8% accuracy. Conclusion DNA aneuploidy and CEA abnormality are correlated to malignant pleuroperitoneal fluids, and can be important auxiliary measures for cytology. Combined FCM-DNA ploidy analysis and cytology can improve the diagnostic accuracy.