快速培养轮状病毒的提纯及电子显微镜下观察

Purification and Observation of Rotaviruses Grown Rapidly in Cell Culture

建立轮状病毒的快速培养和电镜检查的方法。A组轮状病毒SA11株经含10μg/mL蛋白酶的DMEM激活后,在MA104细胞上培养一天,提纯,电镜观察。培养24小时的轮状病毒,提纯后电镜下可观察到大量完整和空心的轮状病毒。结果表明,此法快速培养的SA11病毒,负染后电镜观察很容易看到大量完整轮状病毒。

To investigate a method for the rapid cultivation of rotaviruses. Rotavirus （ SAll strain） was activated with trypsin （ 10μg/mL） ,incoporated trypsin（ 10μg/mL） in the maintence medium and incubated at 37℃ for ld in MA104 cell. The particles of viruses were purified with caesium chloride density gradient centrifugation and observed by EM. There were large amount of antigens, dsRNA and viral particles of SA11 strain rotavirus detected in cells infected. Conclution： A rapid method to produce high yields of rotavirus particles was developed.