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利用组织芯片技术研究乳腺癌中VEGF-C的表达与淋巴管生成的关系及意义

Relationship of VEGF-C expression with lymphangiogenesis and its significance in breast carcinoma:a tissue micoarray study
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摘要 目的探讨乳腺癌中血管内皮生长因子C(VEGF-C)的表达与淋巴管生成的关系及意义。方法构建乳腺癌组织芯片,采用免疫组化SP法检测88例乳腺癌VEGF-C的表达,并用podoplanin标记淋巴管。结果有淋巴结转移组与无淋巴结转移组VEGF-C高表达率差异显著(P<0.05),有淋巴结转移组微淋巴管密度(MLVD)明显高于无转移组(P<0.01),VEGF-C染色低表达组与高表达组MLVD差异极显著(P<0.01)。结论VEGF-C可能通过促进淋巴管生成导致乳腺癌淋巴结转移。 Objective To investigate the relationship and significance of the expression of VEGF-C and lymphangiogenesis in breast cancer on tissue chip. Methods Tissue microarray and immunohistochemistry SP method were applied to detect the expression of VEGF-C and to label the microlymphatic vessels in the tumors by podoplanin staining in 88 cases of breast carcinoma. Results The significant difference in the overexpression of VEGF-C existed between lymph node metastasis group and non-lymph-node-metastasis group （ P 〈 0.05）. The difference of MLVD between VEGF-C lower expression group and VEGF- C overexpression group had statistical significance （P 〈 0.01 ）. Conclusion VEGF-C facilitates lymph node metastasis in breast carcinoma by promoting lymphangiogenesis.
作者 于建宪 韩增磊 纪萍
机构地区 青岛市立医院病理科
出处 《诊断病理学杂志》 CSCD 2008年第1期34-36,共3页 Chinese Journal of Diagnostic Pathology
基金 青岛市科技局重点资助项目(03-NY-14-2)
关键词 乳腺癌 组织芯片 VEGF-C 淋巴管生成 Breast cancer Tissue microarry VEGF-C lymphangiogenesis
分类号 R737.9 [医药卫生—肿瘤]
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参考文献9

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二级参考文献21

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诊断病理学杂志
2008年 第1期

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