摘要
以分离纯化的光系统Ⅱ反应中心D1/D2/Cyt b559复合物为实验体系,在厌氧条件下,观察到Cytb559的光还原,表明Cyt b559能直接从Pheo^-接受电子,而且Cyt b559的光还原是不可逆的。当外加次级电子受体2,6-二甲基苯醌(DMBQ)与D1/D2/Cyt b559复合物重组之后,Cyt b559的光还原被延迟了,此时电子主要通过DMBQ传递,而且还原的Cyt b559在光照后的暗放置中有部分氧化。作者认为不依赖于醌受体的由Pheo^-到Cyt b559的电子传递是一条新的、次要的电子传递路线,它对光系统Ⅱ反应中心起保护作用。
The isolated and purified photosystem Ⅱ(PSⅡ) reaction center Dl/D2/Cyt b559 complex was taken as the experimental system. It was observed that under anaerobic conditions, cy-tochrome b559 (Cyt b559) could be reduced by exposure to strong illumination, suggesting Cyt b559 could accept electrons directly from reduced pheophytin (Pheo-). And the photoreduction of Cyt b559 was irreversible. When the isolated Dl/D2/Cyt b559 complex reconstituted with exogenous secondary electron acceptor 2,6-dimethyl-benzoquinone (DMBQ) , the photoreduction of Cyt b559 was delayed in the function of illumination time. Meanwhile, the electrons transferred mainly through DMBQ and photoreduced Cyt b559 could be partially reoxidized in the dark incubation following illumination. It was concluded that the quinone-independent electron transfer via Cyt b559 was a new, secondary electron pathway, which represented one of the protective pathes for PS II reaction center to dissipate excess excitation energy.
基金
中国科学院重大基金
关键词
光合作用
光系统Ⅱ
反应中心
Cytb559
光还原
Photosystem II reaction center, Dl/D2/Cyt b559 complex, Cyt b559, Photoreduc-tion, Photoprotection
