青藤碱对类风湿关节炎患者成纤维样滑膜细胞增殖及基质金属蛋白酶-3表达的影响

Effects of Sinomenine on Proliferation of Human Fibroblast-like Synoviocyte and Expression of MMP-3 mRNA from Patients with Rheumatoid Arthritis

目的观察青藤碱对体外培养类风湿关节炎患者成纤维样滑膜细胞增殖以及基质金属蛋白酶-3(MMP-3)mRNA含量的影响。方法培养人成纤维样滑膜细胞,分为青藤碱高、中、低剂量组和空白对照组,经青藤碱干预后,用四甲基偶氮唑蓝法测定细胞增殖情况,用半定量RT-PCR方法检测成纤维细胞中MMP-3mRNA的表达。结果经青藤碱干预后的人成纤维样滑膜细胞的增殖率受到显著抑制(P<0.05),其中高剂量组的改变差异尤为显著(P<0.01)。MMP-3的mRNA表达水平较之空白对照组有显著下降(P<0.05),且高剂量组的差异尤为显著(P<0.01)。结论青藤碱可以抑制类风湿关节炎患者成纤维样滑膜细胞的增殖并下调MMP-3的表达,推断这可能是青藤碱治疗类风湿关节炎的一种机理。

Objective To observe the effect of sinomenine on the proliferation of fibroblast-like synoviocytes obtained by digesting synovial tissues with collagens and were divided into four groups： sinomenine high, middle, low concentration and control group. The proliferation of FLS was assessed by methyl-thiazolyl-tetrazolium （MTT） assay and the mRNA expression of MMP-3 was measured by semi-quantitative RT-PCR respectively after treated by sinomenine. Results The rate of FLS proliferation was significantly reduced in groups treated by sinomenine. Compared with control group, the mRNA expression of MMP-3 markly decreased in sinomenine groups （P〈0.05） and especially in sinomenine high group （P〈0.01）. Conclusion Sinomenine can effectively inhibit the proliferation of FLS and reduce the mRNA expression of MMP-3 of FLS in rheumatoid arthritis, that may be a mechanism in therapy of rheumatoid arthritis by sinomenine.