我国首次发现的暗纹节菱孢所致皮肤真菌病的毒理实验及病理分析

MYCOSIS CAUSED BY ARTHRINIUM PHAEOSPERMUM,ITS ANIMAL TOXICITY AND PATHOLOGICAL ANALYSIS

从患者皮损处皮屑及活检组织中分离出的暗纹节菱孢Arthriniumphaeospermum（Corda）Ellis菌株制成混悬菌液，用兔、豚鼠及小鼠（LACA）进行动物毒理实验。表皮直接涂抹法接种，四周后未见皮肤损害；划破表皮涂沫法及皮下注射法接种，两周后实验动物的皮肤都出现浸润性斑块、小给节、皮下脓肿及脱毛的症状，对照组动物正常。再用腹腔注射法接种，小鼠两周后死亡，病解所见，腹膜、肠系膜、腹壁及肝等处，均出现散在的灰白色小脓肿；心、肺、肝、脾及肾均有病变。豚鼠两周后仍存活，当即处死解剖所见与小鼠病解改变相同。对实验动物皮损、腹膜、肠系膜、胃及肝等处的小脓肿组织进行了病理检查，结果发现皮损及器官组织均有明显的病理浸润，由多种细胞构成的浸润性肉芽肿。在病理浸润内可见大小不等的球形、卵圆形、棱形及晶体形的棕色、红色孢子．其病理改变与患者皮损病理改变相一致，其不同点是实验动物有小脓肿，肉芽肿内可见多核巨噬细胞，而在病人皮损中很少见到。

This paper describes the results of animal toxicity test and pathological analysis of Arthrinium phaeospermum. The strain of Arthrinium phaeosprtmum was isolated fromthe skin lesions and the biopsy hssues of patients.The toxicity was tested on rabbits,guinea pigs and mice (LACA) with directly smearing the suspension of isolate, no skinlesion was found in 4 weeks; then with pricking the surface of skin and subcutaneous injection, tWo weeks later the lesion of infiltrative plagues, tubereules nade, subcutaneous abscesses occured on the skin and caused hair loss. When the suspension was injected intothe abdominal cavities of the tested animals, the mice died in two weeks, with small grayabscesses appeared in the peritoneum and mesentery.There are pathogenesis changes on theabdominal wall and liver, spleen, lung, kidney and heart. The guinea pigs were killed aftertwo weeks, and the pathogenesis changes are the same with as those found in the mice.The results showed that apparent pathological infiltrations were seen in the tissues.Thereare brown and red spores of various sizes and shapes in the infiltraction areas withpathological changes which are similar to those found in skin lesion of the patient, butshowed abscess granuloma with multinuclear giant cells which are rarely seen in skinlesiones of the patients.