银屑病患者外周血调节性T细胞的检测

Regulatory T cells in peripheral blood of patients with psoriasis

目的 检测不同类型银屑病患者外周血中调节性T细胞（Treg）的水平。方法 用流式细胞仪和单克隆荧光抗体检测20例寻常性银屑病、15例脓疱性银屑病和10例红皮病性银屑病患者外周血中CD4^＋CD25^bright调节性T细胞百分率，对寻常性银屑病患者中的10例进行了CD4-CD25^hi-int CD127^kw/-调节性T细胞百分率的检测。结果红皮病性银屑病组CD4^＋CD25^hright,调节性T细胞比例高于其他组（P〈0．01）；寻常性银屑病患者组CD4＋CD25^hi-intD127^kw/-调节性T细胞在CD4^＋T细胞中比例为4，39％±0．84％，正常人对照组为3，83％±1．68％，两组进行Mann—Whitney U检验，差异无统计学意义（P〉0．05）。结论 CD4^＋CD25^hright,调节性T细胞可能与红皮病性银屑病的发病有关。

Objective To investigate the levels of regulatory T cells in peripheral blood of patients with different types of psoriasis. Methods Flow cytometric analysis with monoclonal antibody was used to detect the percentage of CD4^＋CD25^bright T cells in CD4^＋ T cells in the peripheral blood of 20 patients with psoriasis vulgaris, 15 patiens with psoriasis pustulosa and 10 patients with erythrodermic psoriasis, as well as the proportion of CD4^＋CD25^hi-intCD127^low/- regulatory T cells in CD4^＋ T cells in the peripheral blood of 10 patients with psoriasis vulgaris, Results The percentage of CD4^＋CD25^bright regulatory T cells was （5.37 ± 1.99）% in patients with erythrodermic psoriasis, significantly higher than that in the healthy controls （P 〈 0.01 ）, patients with psoriasis vulgaris （ P 〈 0.05）, and patients with psoriasis pustulosa （ P 〈 0,05 ）. There was no significant difference in the percentage of CD4^＋CD25^hi-int CD127^klow/- regulatory T cells between patients with psoriasis vulgaris and healthy controls （（4.39± 0.84）% vs, （3,83±1.68）%, P 〉 0.05）. Conclusion CD4^＋CD25^bright regulatory T cells may be related to the pathogenesis of erythrodermic psoriasis.