水稻抗病基因同源序列与抗瘟性的关系

Relationship Between Resistance Gene Analogue and Blast Resistance in Rice

利用11对抗病基因同源序列(RGA)引物扩增43个水稻品种的DNA片段和接种33个稻瘟菌株测定它们的抗病性。结果表明,抗病性聚类结果与DNA扩增条带聚类结果基本一致,两者相关系数达0.6117(α=0.01),即利用RGA分析品种的抗病性具有很好的代表性,但相关性在引物间不同,从0.1701到0.5305。结合多态性分析,5对引物S1/AS3、S1INV/S2 INV、XLRR For/XLRR Rev、Pto-Kin 1 IN/Pto-Kin 2 IN、NLRR For/NLRR Rev可用于水稻品种的抗稻瘟病性分析,它们扩增的DNA条带数据与抗瘟性间达极显著相关。此外,除高感品种丽江新团黑谷和CO39两个品种未被聚类到不同的亚种外,RGA扩增的DNA条带还能区分水稻的籼粳亚种类型。

DNA fragments of 43 rice varieties were amplified with 11 pairs of primers designed based on resistance gene analogue （RGA） of plants and blast resistance of the varieties was identified by inoculation with 33 isolates collected from Yunnan Province, China. Clustering results revealed significant correlation between the resistance and DNA bands with a correlation coefficient of 0. 6117 （α=0.01）, indicating that the resistance analysis based on RGA-PCR bands was coincided with that based on inoculation. However, the correlation coefficient, ranged from 0. 1701 to 0. 5305 depending on the primers. The 5 pairs of primers, S1/AS3, $1 INV/S2 INV, XLRR For/XLRR Rev, Pto-Kin 1 IN/Pto-Kin 2 IN, NLRR For/NLRR Rev could be applied to blast resistance identification under consideration of their band numbers and polymorphism. Besides, the 11 pairs of primers could classify indica and japonica rice very well except two highly susceptible varieties, CO39 and Lijiangxintuanheigu.