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重组大肠杆菌产右旋糖酐蔗糖酶的培养基优化 被引量：1

Medium Optimization for Dextransucrase Production by Recombinant Escherichia coli
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摘要 研究了培养基中葡萄糖、酵母浸膏、Ca2+浓度及无机离子类型等对重组大肠杆菌产右旋糖酐蔗糖酶的酶活力影响。在优化的培养基条件下,右旋糖酐蔗糖酶酶活力达26.6u/ml。使用该重组酶催化制备右旋糖酐,在8h内底物转化率达82%,右旋糖酐分子量高于355k。 Culture medium optimization for recombinant Escherichia coli producing dextransucrase was investigated, including the concentrations of glucose, yeast extract and Ca^2＋, as well as the influence of inorganic ions. Under the optimal culture conditions, the activity and biotransformation yield of recombinant dextransucrase could reach to 26.6 u/ml and 82% in 8h and the molecular weight of dextran was over 355k.
作者 张洪斌 胡又佳 朱春宝 朱宝泉 王雅洁
机构地区 上海医药工业研究院 合肥工业大学制药工程系
出处 《中国医药工业杂志》 CAS CSCD 北大核心 2008年第3期179-181,共3页 Chinese Journal of Pharmaceuticals
基金 上海市科委资助项目(07DZ22002) 安徽省高等学校青年教师科研资助计划项目(2005JQ1004)
关键词 右旋糖酐蔗糖酶 重组大肠杆菌 培养基 优化 dextransucrase recombinant Escherichia coli culture medium optimization
分类号 Q555.4 [生物学—生物化学]
  • 相关文献

参考文献9

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共引文献31

同被引文献18

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二级引证文献2

中国医药工业杂志
2008年 第3期

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