热疗联合放化疗对人肺腺癌耐药细胞株A549／DDP的协同杀伤作用

The synergistic effects of hyperthermia combined with radiochemotheraPx on muitidrug-resistant humam lung adenocarcinoma cell line A549/DDP

目的观察热疗联合放化疗对人肺腺癌耐药细胞株A549／DDP的协同杀伤作用，为临床上应用热放疗和热化放疗联合治疗非小细胞肫癌提供实验依据。方法采用CCK-8法和克隆形成实验检测热放疗或热放化疗对A549／DDP细胞抑制率和克隆形成率的影响；采用流式细胞仪检测热放疗或热放化疗联合作用后A549／DDP细胞的凋亡现象；采用光镜和透射电镜观察细胞形态学改变。结果热放疗尤其是热放化疗联合对A549／DDP细胞有明显抑制作用（P〈0．01）；流式细胞仪显示热放疗或热放化疗有诱导A549／DDP细胞凋亡作用（P〈0．01）；电子显微镜观察到细胞核固缩、变形，染色质浓聚、边集等凋亡现象。结论热放疗或热放化疗能诱导A549／DDP细胞凋亡。

Objective To observe the synergistic effects of hyperthermia and radiochemotherapy on muhidrug-resistant human lung adenocarcinoma cell line A549/DDP. Methods The muhidrug-resistant human lung adenocarcinoma cell line A549/DDP was cultivated and then subject to thermoradiotherapy or thermoradiotherapy in combination with chemotherapy. The A549/DDP cell inhibition ratio and clone formation rate were monitored with CCK-8 assay and clone formation experiment. Flow cytometry was used to detect the A549/DDP apoptosis phenomenon. Light microscope and transmission electron microscope were used for morphologic observation of the cells. Re- suits Thermoradiotherapy especially hyperthermo-chemo-radiotherapy inhibited AS49/DDP cell proliferation. Flow cytometry showed thermoradiotherapy combining with or without chemotherapy could induce AS49/DDP apoptosis. Electron microscopy demonstrated features of apoptosis, such as karyopyknosis ,deformation, karyorrhexis and chromatin accumulation and aggregation. Conclusion Thermoradiotherapy and hyperthermo-chemo-radiotherapy could induce AS49/DDP apoptosis.