c—ski在IgA肾病模型大鼠肾脏组织中的表达及意义

Expression and significance of c-Ski in lgA Nephropathy model rats

目的观察c-Ski在IgA肾病大鼠肾脏组织的表达，分析其与IgA在肾脏沉积的相关关系，探讨IgA肾病的发病机制。方法建立IgA肾病大鼠模型，分为IgA肾病模型组、强的松干预组和正常对照组。免疫荧光观测IgA在肾组织的沉积，免疫组化法检测c-ski在肾组织的表达。结果与正常对照组比较，IgA肾病模型组c-ski表达降低，且与IgA沉积呈负相关，强的松干预组c-Ski表达较模型组升高。结论c-Ski是IgA肾病的保护性因子，其表达降低可能与与IgA沉积有关。

Objective To investigate the expression of c-Ski in IgA Nephropathy and explore its significance in the mechanism of the disease. Method 30 rats were randomly divided into 3 groups： IgA Nephropathy group （ n = 10） , Prednisone group （ n = 10） and normal control group （ n = 10）. The sediment of IgA was evaluated with immunofluorescence, the expression of c-Ski in tissues of IgA Ne- phropathy were detected with immunohistochemistry. Results The expression of c-Ski in IgA Nephropathy group was significant lower than that in normal control group. The correlation between IgA sediment and c-Ski was negative, Condusion c-Ski is one of protective factors of IgA Nephropathy and IgA sediment in the renal tissue may be the causation of decrease.