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人胚胎骨髓间充质干细胞的体外培养 被引量：3

In Vitro Cultivation of Human Fetal Bone Marrow Mesenchymal Stem Cells
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摘要 目的摸索体外培养人胚胎骨髓间充质干细胞的方法。方法采用含5%胎牛血清的MEM(modified Eagle's medium)培养基培养人胚胎骨髓间充质干细胞,用流式细胞仪检测培养获得的细胞中人胚胎骨髓间充质干细胞含量。结果培养获得的细胞中人胚胎骨髓间充质干细胞含量达93%。结论本研究方法培养获得的人胚胎骨髓间充质干细胞纯度高,细胞活性好。 Objective To explore the cultivation of human fetal bone marrow mesenchymal stem cells （MSC）. Methods MSC were cultured in the modified Eagle＇s medium （MEM with 5% fetal bovine blood serum）. The purity of MSC acquired by our method was detected by flow cytometer. Results The purity of MSC was over 93%. Conclusion It＇s a good way to cultivate MSC in vitro by MEM.
作者 陈焱 陈耀凯 王宇明 夏杰
机构地区 解放军 第三军医大学西南医院感染科
出处 《华南国防医学杂志》 CAS 2008年第1期1-3,共3页 Military Medical Journal of South China
基金 国家自然科学基金项目(30370391)
关键词 人胚胎骨髓问充质干细胞 体外培养 Marrow mesenchymal stem cell Cultivation
分类号 R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献11

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共引文献22

同被引文献21

  • 1王晓云,郭霞,钱忠明.骨组织中降钙素基因相关肽阳性神经的分布及生理作用[J].中华骨科杂志,2005,25(3):185-188. 被引量：14
  • 2周文武,胡建国,杨进福,林玲,马燕琳,周新民,唐滔.血管内皮细胞生长因子基因转染骨髓间充质干细胞后心肌移植对促进心肌缺血大鼠心功能恢复的效果评价[J].中国临床康复,2006,10(5):42-45. 被引量：3
  • 3周瑞祥,孙圣刚.大鼠骨髓间质多能成体祖细胞系统移植治疗脑缺血性损伤的研究[J].中风与神经疾病杂志,2007,24(2):164-166. 被引量：1
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引证文献3

二级引证文献14

华南国防医学杂志
2008年 第1期

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