摘要
目的摸索体外培养人胚胎骨髓间充质干细胞的方法。方法采用含5%胎牛血清的MEM(modified Eagle's medium)培养基培养人胚胎骨髓间充质干细胞,用流式细胞仪检测培养获得的细胞中人胚胎骨髓间充质干细胞含量。结果培养获得的细胞中人胚胎骨髓间充质干细胞含量达93%。结论本研究方法培养获得的人胚胎骨髓间充质干细胞纯度高,细胞活性好。
Objective To explore the cultivation of human fetal bone marrow mesenchymal stem cells (MSC). Methods MSC were cultured in the modified Eagle's medium (MEM with 5% fetal bovine blood serum). The purity of MSC acquired by our method was detected by flow cytometer. Results The purity of MSC was over 93%. Conclusion It's a good way to cultivate MSC in vitro by MEM.
出处
《华南国防医学杂志》
CAS
2008年第1期1-3,共3页
Military Medical Journal of South China
基金
国家自然科学基金项目(30370391)