有机介质对2,3-二氨基吩嗪的荧光增敏作用研究

Studies on the Fluorescence Enhancement of Organic Solvents on 2, 3-Diaminophenazine

研究了有机介质对漆酶催化氧化邻苯二胺的产物──2，3-二氨基吩嗪（DAP）的光谱性质的影响，结果表明；极性有机介质对其吸收光谱影响较小，但对其荧光具有强烈的增敏作用，荧光量子效率可提高近30倍．据此，建立了有机介质增敏荧光测定DAP的新方法．进一步研究表明：有机介质对2，3-二氨基吩嗪的荧光增敏原因在于其增溶作用、供电子效应及对DAP分子吸光性能的增强．

The effects of various organic solvents on spectral characteristics of 2, 3-diaminophenazine (DAP), which is an oxidized product of o-phenylenediamine catalyzed bysome oxidase, were investigated. It has been found that there is a little effect on the absorption features of the product by strong polar organic solvents. However, the fluorescence ofthe product is greatly enhanced by strong polar organic solvents, and the quantum yield canbe increased about 3O-fold by organic medium. Further experlmental results show that fluorescence enhancement of organic solvent on DAP are due to the solubility increasing actionand electron donating effect.