摘要
采用示波极谱法和循环伏安法研究了3公铁离子Fe（Ⅲ）与阿霉素（ADM）配合物的电化学特性．在生理pH条件下，Fe（Ⅲ）与ADM形成2：1的稳定配合物，Fe（Ⅲ）的配位使ADM在更负的电势下才能还原，这与其心脏毒性减轻密切相关．采用线性扫描、吸收光谱和凝胶电泳法研究了ADM－Fe（Ⅲ）配合物与DNA的结合作用，ADM－Fe（Ⅲ）配合物仍保留了ADM插入结合的特征，并形成一种稳定的DNA－Fe（Ⅲ）－ADM的三元结合物．
Electrochemistry characteristics of adriamycin-Fe(Ⅲ) complex was studied by the methods of osillopolarograph and cyclic voltammetry. At physiological pH, Fe (Ⅲ) binds with ADM to form a complex of 2: 1 stoichiometry. ADM was reduced at a more negative potential when it was complexed with Fe(Ⅲ), which is close related to the low cardiac toxicity of the complex. The interaction model of ADM-Fe(Ⅲ) complex with DNA was studied by the methods of spectroscopy, electrochemlstry and electrophoresis. The complex preserves the DNA intercalation characteristics, and binds to DNA to form a ternary complex composed of DNA, Fe(Ⅲ) and ADM.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
1997年第5期671-675,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金
山西省自然科学基金
