摘要
本文采用反相离子对色谱测定了生物发酵液中的七种水溶性维生素。提出了用自行装填的酸性氧化铝(0.19~0.15mm)前置柱,和5%乙酸洗脱样品的前处理方法;并就有关的色谱条件进行了选择。色谱柱为NovaPakC18;流动相A液为0.05mol/L庚烷磺酸盐(PICB7),冰乙酸调pH=2.5;B液为甲醇+三乙胺(100+0.5);梯度洗脱;UV254nm、290nm、360nm同时测定。线性范围为0.1~10μg,各种维生素的RSD在1.2%~3.0%之间(VitaminB12除外),回收率大于90%。
A HPLC method for determination of water soluble vitamins in the solution of biological fermentation is reported.The samples were processed by a per column packed with acidic aluminum oxide before injected in chromatographic column.A NovaPak C 18 as the chromatographic column,0.05 mol/L PIC B7 solution adjusted pH 2.5 by glacial acetic acid (A) and methanol+triethylamine(100+0.5)(B) as mobile phase for gradient elution were adopted in HPLC analysis.Seven kinds of vitamins are detected at UV 254 nm,290 nm and 360 nm with a linear range of 0.1~10 μg,RSD of 1.2%~3.0%(except Vitamin B 12 ).The recoveries all are more than 90%.
出处
《分析测试学报》
CAS
CSCD
1997年第3期83-85,共3页
Journal of Instrumental Analysis