碳氮比和光强对小球藻合成虾青素的影响

Effects of C/N Ratio and Light Intensity on the Production of Astaxanthin by Chlorella zofingiensis

本文研究了碳氮比和光强对小球藻Chlorella zofingiensis合成虾青素的影响。结果表明,随着碳氮比的增加,虾青素含量提高,但是过高的碳氮比限制藻细胞的生长。当碳氮比为133时,虾青素的产量最大,达到9.19mg/L。高光诱导在高碳氮比基础上进一步提高虾青素的含量及产量。200μmol/m2s光强在没有严重影响小球藻生长的同时,明显提高虾青素含量,最大虾青素产量达到12.52mg/L。文章对不同诱导条件下虾青素的合成机理以及利用异养自养相结合的方法提高虾青素产量的可能性进行了讨论。

The effect of C/N ratio and light intensity on the astaxanthin accumulation of Chlorella zofingiensis was studied in this research. C/N ratio showed obvious effect on the astaxanthin accumulation. Astaxanthin content rapidly increased with the enhancement of C/N ratio, while the alga growth was inhibited, the biomass was far lower than the control. The maximum astaxanthin yield of 9.19 mg/L was obtained at a C/N ratio of 133. High light could farther enhance astaxanthin content and yield. A light intensity of 200 μmol/m^2.s could induce maximum astaxanthin yield to 12.52 mg/L without serious inhibition in the biomass. Be sides, the mechanisms of different induction were discussed, and the method combined heterotrophic and phototrophic culture was proposed to enhance astaxanthin production by C. zofingiensis.