摘要
在明确链霉素对农抗TS99产生菌Streptomyces fungicidicus YH04孢子的致死浓度为1.2μg/mL的基础上,以链霉素致死浓度为选择压力,采用不同剂量的紫外线照射对菌株孢子进行诱变处理,获得了大量的链霉素抗性基因突变株,进而从中筛选到发酵效价较出发菌株提高60%以上,且遗传稳定性良好的高产菌株Streptomyces fungicidicus YH9407。
Streptomycin-resistant mutants were obtained from original strains YH04 treated by UV （253 nm, 30 W, 45 s or 60 s） under the selection pressure of resistance to streptomycin （the lethal concentration is 1.2 μg/mL）. Streptomyces Strain YH9407 with high antibiotic yield and genetic stability was obtained from streptomycin-resistant mutants. The yield of antibiotic TS99 is more than 60% higher than that of the original strain.
出处
《微生物学通报》
CAS
CSCD
北大核心
2008年第3期384-388,共5页
Microbiology China
基金
国家烟草专卖局科技项目(No.国烟科[2002]187号)
关键词
衣用抗生素
紫外诱变
链霉素致死突变
诱变育种
Agriantibiotic, Ultraviolet induced, Streptomycin lethal mutate, Mutation breeding
