马尾松化学热磨机械浆酶法改性研究

Modification of Masson Pine Chemo-thermo-mechanical Pulp by Rhus Laccase Treatment

利用漆树漆酶/介体体系对马尾松化学热磨机械浆进行生物酶处理及后续H2O2漂白,探索漆酶生物改性最佳工艺条件。结果表明,在漆酶用量为2.0 U/g,2,2’-连氮-二(3-乙基噻唑-6-磺酸)(ABTS)用量0.5%,酶处理温度50℃,酶处理时间60 min时,与对照浆相比,纸浆白度可提高3.0%ISO,抗张指数、撕裂指数分别提高22.5%、7.9%。纤维长度、粗度分析及扫描电镜谱图显示,漆酶处理只发生在纤维表面,有利于表面木素的降解溶出,从而提高了纸浆的白度和可漂性。

The Masson pine unbleached chemo-thermo-mechanical pulp （CTMP） was treated with laccase in the presence of ABTS, and then bleached with hydrogen peroxide （H2O2）. It was found that the optimum conditions for laccase-mediator-system （LMS） treatment were： the charge of laccase 2.0 U/g（pulp）, ABTS 0.5%（pulp）, temperature 50℃, reaction time 60 min. At the optimum conditions, compared to the control pulp, the brightness of LMS treated pulp could be increased by 3.0%ISO, the tensile index and tear index could be increased by 22.5%, 7.9% respectively. The average length, coarseness and SEM of LMS modified fiber indicted that laccase action mainly occurred at the surface of fiber, in favor of dissolve surface lignin thus promoting the brightness and bleachability of pulp.