人乳头瘤状病毒16型与新疆哈萨克族食管鳞癌的相关性研究

Correlation between HPV16 infection and Squamous Cell Carcinoma of Esophagus in kazakh’s of Xinjiang,China

目的:探讨新疆哈萨克族食管鳞癌患者中人乳头瘤状病毒(HPV)16型的检出率。方法:采用半巢式PCR技术检测150例新疆哈萨克族食管癌石蜡包埋组织、40例正常食管粘膜活检组织中HPV16型,分析其与新疆哈萨克族食管癌发生的相关性。结果:在新疆哈萨克族食管癌组织中,HPV16型检出率为38.7%(58/150),与对照组10%(4/40)相比,差异有统计学意义(χ2=11.805,P<0.05)。结论:HPV16型感染与新疆哈萨克族食管癌的发生存在一定的相关性。

Objective：To evaluate the rate of Human papillomavirus （HPV）16 infection in esophageal carcinoma of kazakh＇s in Xinjiang. Methods： Using Specific primer for HPV16E6, We detected the prevalence of a HPV16E6 gene in tumor tissues from 150 ESCC cases in formalin-fixed, parafin-embedded and 40 nomal samples by used semi-nested polymerase chain reaction. Results： 58 （38.7 % ） of the 150 ESCC samples was positive for the HPV 16E6 gene. Compared with ESCC gourp,the positive rate of HPV 16E6 gene were 10% （4/40）in control gourp. In statistical analysis, the diference was significant. Conclusion：HPV DNA infection was closely related to esophageal carcinoma of Kazakh in Xinjiang.