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毕赤酵母高效表达外源基因的研究进展 被引量：1

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摘要 巴斯德毕赤酵母(Pichia pastoris,Pp)表达系统是目前分子生物学领域中用于表达重组蛋白的标准工具之一[1,2]。酵母被认为是一个很有前途的真核表达系统而受到广泛的关注。本文综述了Pp在生物学特性、遗传学特征、载体、基因整合、胞内表达和分泌表达、影响外源蛋白在Pp表达系统中高效生产因素的研究进展。
作者 吴万贵 袁俐 王仙
机构地区 石河子大学医学院病原生物学与免疫学教研室
出处 《农垦医学》 2008年第1期56-58,共3页 Journal of Nongken Medicine
关键词 毕赤酵母 表达系统 研究进展
分类号 Q7 [生物学—分子生物学]
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参考文献9

  • 1Cregg J M, Cerehino J L, Shi J, et al. Reombinant protein express in Pichia Pastoris. Mol Biotechnol, 2000,16( 1 ) : 23 - 52
  • 2Cereghino J L, Cregg J M. Heterologous protein expression in the methylotro-phic yeast Pichia pastoris. FEMS Microbiol Rev,2000,24 (1):45-66
  • 3Heckney R C. Recent developments in heterologous protein production in Eschefichia coll. Trends Bioteeh, 1994,12 ( 11 ) :456- 463
  • 4Bruel C, Cha K, Reeves P J, et al. Rhodopsin kinase: expressionin mammalian cells and a two - step purification. Proc Natl Acad Sci USA, 2000,1997(7) :3004 - 3009
  • 5Honenberg C P, Gellissen G. Production of recombinant proteins by methylotrophic yeasts. Curt Opin Biotechnol, 1997,8 (5) : 554- 560
  • 6王勇,刘哲伟.毕赤酵母表达外源基因研究进展[J].微生物学免疫学进展,2004,32(1):62-66. 被引量：24
  • 7郑立运,方先珍.酵母表达系统最新研究进展[J].郑州牧业工程高等专科学校学报,2005,25(4):250-253. 被引量：10
  • 8Trinh L , Noronha S B, Fannon M, et at. Recovery of mouse endostatin produced by Pichia pastoris using expanded bed adsorption. Bioseparation, 2000,9(4) :223 - 230
  • 9Cereghino J L,Cregg J M.Heterologous protein expressive in the methylotrophic yeast Pichia pastoris .FEMS Microbiol Rev,213130,24(1) :45 - 66

二级参考文献21

  • 1Shen S, Sultar G, Jeffries T W, et al. A strong nitrogen source regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris[J].Gene, 1998,216:93-102.
  • 2Liu H, Tan X, Russell KA, et al. A gene required for peroxisome biogenesis in Pichia pastoris , encodes a peroxisomal membrane protein involved in protein import[J]. J Biol Chem,1995,270:10940-10951.
  • 3Sears I B, O'Connor J, Rossanese O W, et al. A versatile set of vectors for constitutive and regulated gene expression in Pichia pastoris[J]. Yeast,1998,14: 783-790.
  • 4Vassileva A, Chugh D A, Swaminathan S, et al. Effect of copy number on the expression levels of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris[J]. Protein Expr Purif,2001,21:71-80.
  • 5Clare J J, Rayment F B, Ballantine S P, et al. High level expression of tetanus toxin fragment C in Pichia pastoris strains containing multiple tandem integrations of the gene[J]. Biotechnology,1991,9:455-460.
  • 6Scorer C A, Clare J J, McCombie W R, et al. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high level foreign gene expression[J]. Biotechnology,1994,12:181-184.
  • 7Higgins D R, Busser K, Comiskey J, et al.Small vectors for expression based on dominant drug resistance with direct multicopy selection[J]. Methods Mol Biol,1998, 103:41-53.
  • 8Clare J J, Romanos M A, Rayment F B, et al. Production of mouse epidermal growth factor in yeast:high2level secretion using Pichia pastoris strains containing multiple gene copies[J].Gene,1991,105:205-212.
  • 9Brocca S, Schmidt Dannert C, Lotti M, et al. Design,total synthesis,and functional over expression of the Candida rugosalip1 gene coding for a major industrial lipase[J]. Protein Sci,1998,7:1415-1422.
  • 10Kjeldsen T, Pettersson A F, Hach M. Secretory expression and characterization of insulin in Pichia pastoris[J]. Biotechnol ApplBiochem, 1999,29:79-86.

共引文献30

同被引文献20

  • 1THOMAS Vogl, ANTON Glieder. Regulation of Pichia pastoris promoters and its consequences for protein production [J]. New Biotechnology, 2013,30(4) : 385-404.
  • 2SHA C, YU X W, LIN N X, et al. Enhancementof lipase r27RCL production in Pichia pastoris by regulating gene dosage and co-expression with chaperone protein disulfide isomerase [J]. Enzyme and Microbial Technology, 2013,53(6/7): 437-443.
  • 3KHAN M A, HASSAN N, AHMAD N, et al. Studies to analyse the relationship between IFNa2b gene dosage and its expression, using a Pichia pastoris-based expression system [J]. Yeast, 2014, 31 (1) : 13-28.
  • 4HOHENBLUM H, BORTH N, MATTANOVICH D. Assessing viability and cell-associated product of recombinant protein producing Pich ia pastoris with flow cytometry[J]. Journal of Biotechnology, 2003, 2(3) 281-290.
  • 5HOHENBLUM H, GASSER B, MAURER M, et al. Effects of gene dosage, promoters, and substrates on unfolded protein stress of recombinant Pichia pastoris [J]. Biotechnology and Bioengineering, 2004, 5(4) : 367-375.
  • 6RON D, WALTER P. Signal integration in the endoplasmic reticulum unfolded protein response [J]. Nature Reviews Molecular Cell Biology, 2007,8(7) .. 519-529.
  • 7GUERFAL M, RYCKAERT S, JACOBS P P, et al. The HAC1 gene from Pichia pastoris: Characterization and effect of its overexpression on the production of secreted, surface displayed and membrane proteins [J]. Microbial Cell Factories, 2010,9.. 49-61.
  • 8GASSER B, MAURER M, GACH J, et al. Engineering of Pichia pastoris for improved production of antibody fragments [J]. Biotechnology and Bioengineering, 2006,94(2) : 353-361.
  • 9PAYNE T, FINNIS C, EVANS L R, et al. Modulation of chaperone gene expression in mutagenized Saccharomyces cerevisiae strains developed for recombinant human albumin production results in increased production of multiple heterologous proteins [J]. Applied and Environmental Microbiology, 2008, 4(24) .. 7759-7766.
  • 10HEMMERICH J, ADELANTADO N, BARRIGON J M, et al. Comprehensive clone screening and evaluation of fed-batch strategies in a micro bioreactor and lab scale stirred tank bioreactor system: Application on Pichia pastoris producing Rhizopusoryzae lipase [J]. Microbial Cell Factories, 2014, 1(3) .. 36-52.

引证文献1

农垦医学
2008年 第1期

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