CD4^＋CD25^＋调节性T细胞在系统性红斑狼疮患者外周血的表达

CD4^＋CD25^＋ Regulatory T cells in peripheral blood of patients with systemic lupus erythematosus

目的检测系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中CD4^＋CD25^high细胞占CD4^＋T细胞的比例及FOXP3基因mRNA表达，探讨其在SLE发病中的意义。方法选择SLE患者77例，正常对照25名。流式细胞法分析CD4^＋CD25^high细胞占CD4^＋T细胞的比例；实时定量聚合酶链反应（PCR）方法检测FOXP3 mRNA表达。结果活动期SLE患者CD4^＋CD25^high百分率下降，FOXP3基因表达下降；活动期SLE患者FOXP3基因表达与SLEDAI呈负相关，与红细胞沉降率、补体无相关。结论CD^4＋D25^high百分率下降，FOXP3基因表达降低提示调节性T淋巴细胞的数量减低及功能下降在SLE的发病中可能起到重要作用。

Objective The present study is aimed to characterize and quantify CD4^＋CD25^＋ regulatory T cell population in the peripheral blood of patients with systemic lupus erythematosus （SLE）. Methods Peripheral blood mononuclear cells （PBMC） were isolated from 42 active SLE patients, 35 stable SLE patients and 25 healthy controls. CD4^＋CD25^high T cells were analyzed by flowcytometry, and mRNA expression of FOXP3 was determined by real-time PCR. Results PBMC from patients with active SLE showed lower popu-lations of CD4^＋CD25^high compared with the controls. The active SLE patients also expressed reduced levels of FOXP3 mRNA. Expression of FOXP3 mRNA showed negative correlation with SLEDAI, but not correlated with ESR,C3 ,C4. Conclusion These results demonstrate that deficiency of CD4^＋CD25^high T cell may play an important role in the pathogenesis of SLE.