HPLC法测定不同产地三白草中三白草酮的含量

Determination the Content of Sauchinone of Saururus Chinensis(Lour.)Bail.from Different Areas by HPLC

目的:建立中药材三白草中三白草酮的含量测定方法。方法:岛津ODS-C_(18)(150 mm×4.6 mm,5μm)色谱柱,流动相:乙腈-水(60:40),检测波长244 nm。结果:三白草酮含量测定线性范围为0.2—1.20μg,平均回收率为100.1%,RSD为1.88%。结论:方法可靠,简单可行,为控制三白草内在质量提供了科学依据。

Objective： To set up a method for determination the content of Sauchinone of Saururus chinensis. Method： The ODS- C18 column（ 150 mm × 4.6 mm,5 μm）was used, mobile phase was Acetonitrile-water（60：40）, detection wavelength was at 244 nm. Result： The standard curve was linear within a range of 0.20 - 1.20 μg. The average recovery and RSD were 100.1% and 1.88 ,respectively. Conclusion： This method is accurate,reliable and can provid a scientic index for quality control of Saururus chinensis.