摘要
为准确鉴别海桑属植物,采用ISSR(inter-simple sequence repeat)分子标记技术,对6种海桑属植物(海桑、拟海桑、杯萼海桑、海南海桑、卵叶海桑及无瓣海桑)基因组DNA进行PCR扩增。建立了海桑属植物ISSR标记的标准程序和海桑属各物种的DNA指纹数据库。采用的11个引物共产生71个物种特征性标记,其中无瓣海桑23个,海南海桑16个,海桑15个,杯萼海桑6个,拟海桑6个,卵叶海桑5个。运用这些特征性标记,可迅速区分海桑属植物。研究表明ISSR技术是在分子水平上鉴定海桑属植物的一种行之有效的方法。
The utility of inter-simple sequence repeat （ISSR） DNA polymorphism to distinguish 6 taxa （Sonneratia caseolaris , S. paracaseolaris , S. alba, S. hainanensis, S. ovata, S. apetala ） in the genus Sonneratia was investigated. The ISSR standardized procedure and the fingerprints database of Sonneratia species were established. 11 ISSR primers used produced 71 species-specific markers for the six Sonneratia species, including 23 specific to S. apetala, 16 specific to S. hainanensis, 15 specific to S. caseolaris, 6 specific to S. alba, 6 specific to S. paracaseolaris and 5 specific to S. ovata. According to these special markers, the six Sonneratia species could be identified quickly and accurately. It was shown that the ISSR technique is a reliable and effective tool to differentiate the Sonneratia species.
出处
《植物研究》
CAS
CSCD
北大核心
2008年第2期205-210,共6页
Bulletin of Botanical Research
基金
教育部高等学校博士学科点专项科研基金资助项目(20010558004)
关键词
海桑属
ISSR
分子鉴定
Sonneratia
ISSR
molecular identification
