极大螺旋藻生物富钙作用的研究

Studies on Calcium Bioaccumulation of Spirulina Maxima

为开发一种新型功能食品——富钙螺旋藻,分别以透析和直接添加两种方式向螺旋藻培养液中加入CaCO3、CaSO4以及贝壳等钙盐,研究极大螺旋藻对钙的生物富集作用及在相应条件下的生长状况。结果表明:两种培养方式下,极大螺旋藻的生长情况尚好,但或多或少受到一定程度的抑制,尤其是直接加入CaCO3和CaSO4的螺旋藻。螺旋藻对钙的富集作用并不随培养液中游离钙离子浓度的增加而增加。添加的几种钙盐中,直接加入CaCO3和CaSO4的螺旋藻钙含量高,分别是对照的14倍和23倍,其次是加入贝壳,其钙含量是对照的8～10倍,而采用透析方法加入CaCO3和CaSO4的螺旋藻,其钙含量与对照相差无几,甚至比对照还低。因此,直接加入粉末状含钙化合物,能提高螺旋藻对钙的亲和力,强化其对钙的富集作用。

In order to develop a new kind of function food, the growth and calcium bioaccumulation of Spirulina maxima were studied in the present paper by adding CaCO3, CaSO4 and shell into Zarrouk culture by dialysis and direct addition methods respectively. The results showed that growth of S.maxima was not too bad, although it was inhibited to some extent, especially those of S.maxima directly added CaCO3, CaSO4. Calcium bioaccumulation of S.maxima was not strengthened with the [Ca^2＋] increasing in culture, but enhanced much more when directly adding calcium compound such as CaCO3 and CaSO4 into the culture. The highest calcium contents in S.maxima were about 14 and 23 times as high as control respectively when directly adding CaCO3 and CaSO4 into the culture. The second high calcium content was about 8～10 times as high as control when adding shell into the culture. When adding CaCO3 and CaSO4 into the culture by dialysis, the calcium contents were almost as high as control, even less than the control. Conclusion： direct adding method could increase Spirulina affinity to calcium, therefore improve calcium bioaccumulation of S.maxima.