聚γ-谷氨酸延缓磷酸钙沉淀及螯合钙离子的研究

Effects of Poly-γ-glutamic Acid on Retarding Calcium Phosphate Depositing and It's Chelation with Calcium Ion

研究表明聚γ-谷氨酸(γ-PGA)有延缓磷酸钙沉淀形成的能力,在γ-PGA添加量达到200mg/L的条件下,体系中减少磷酸钙沉淀形成率达83.4%,延缓达到最大沉淀量所需时间20min。同时还研究了γ-PGA与钙离子螯合形成螯合钙的条件,结果显示在温度37℃、pH6、3%γ-PGA、羧钙比2.5:1的条件下螯合率最高,螯合反应在15min完成;螯合物的钙含量为11.06%。γ-PGA能与钙离子形成可溶性钙,可开发成一种新型的补钙剂。

The ability of poly- γ-glutamic acid（γ-PGA）to retard calcium phosphate depositing was investigated in the study. When 200 mg/L γ-PGA was added, it remarkably reduced the calcium phosphate depositing by 83.4%, and retarded the time of achieving the highest depositing to 20 min. The chelation conditions of γ-PGA with calcium ion such as pH, temperature and the concentration of γ-PGA were studied. Results showed that the maximum yield of chelation was gained under the condition of temperature 37℃, pH 6.0, 3% γ-PGA and the molar ratio of carboxyl group of γ-PGA to calcium ion 2.5：1, then the chelation course was finished within 15 min and the content of calcium was 11.06% in the chelate complex. It illustrated that γ-PGA was able to chelate with calcium ion and worth exploiting as a new calcium supplemental agent.