灰毡毛忍冬提取纯化物抗氧化性研究

Study on Extraction and Purification of Chlorogenic Acid from Flos Lonicerae Flowers and Its Antioxidation Properties

目的:研究灰毡毛忍冬提取纯化物的抗氧化性。方法:采用超氧阴离子自由基体系、羟基自由基体系和Oyaizu法,对灰毡毛忍冬提取纯化物的抗氧化活性进行研究,并与抗坏血酸(VC)进行比较。结果:灰毡毛忍冬提取纯化物对几种自由基均有不同程度的清除作用,其对超氧阴离子清除作用不明显,比抗坏血酸要弱,最大清除率只有47.71%,并没有超过50%,而灰毡毛忍冬提取纯化物对羟自由基的清除能力以及对Fe3+的还原能力均要明显强于抗坏血酸,分别达到了82.41%(清除率)和0.811(吸光度)。结论:灰毡毛忍冬提取纯化物对羟自由基具有较强的清除能力,对Fe3+具有较好的还原作用。清除作用和还原能力与绿原酸的浓度呈正相关性。除对O2·的清除能力比VC稍弱外,在清除·OH能力和还原力方面均较VC的能力强。

Using the superoxide radical system, hydroxyl radical system and the method of Oyaizu, the antioxidation activities of extraction and purification of chlorogenic acid from Flos Lonicerae flowers were studied and compared with VC. The results showed that extraction and purification of chlorogenic acid from Flos Lonicerae flowers had different scavenging effects in these radical systems. The scavenging capability of extraction and purification of chlorogenic acid from Flos Lonicerae flowers to superoxide radical was not obvious, the scavenging capacity was lower than VC, and the scavenging ratio was 47.71%. But the scavenging capacity of chlorogenic acid to hydroxyl radical and deoxidizing Fe^3＋ was superior than VC, 82.41%（ scavenging ratio） and 0.811 （absorbency） respectively. Chlorogenic acid from Flos Lonicerae had strong capacity to scavenge ·OH, and also had the strong reducing power. The scavenging capacity and the reducing power presented positive relatively with concentration of the chlorogenic acid. Although the scavenging capability of chlorogenic acid to O2^＋ was lower than VC, the scavenging capacity of chlorogenic acid to hydroxyl radical and deoxidizing Fe^3＋ was superior than VC.