莜麦麸中β-葡聚糖提取工艺的优化

Optimization of Process Conditions of Extracting β-glucan from Naked Oat Bran

以莜麦麸为原料提取β-葡聚糖,系统研究了原料处理、去除淀粉、蛋白质及醇析等关键步骤对β-葡聚糖得率和纯度的影响,通过单因素试验及正交试验优化了β-葡聚糖提取工艺条件。采用该工艺得到的β-葡聚糖纯度可达到90%以上。

The β-glucan was extractted from naked oat bran, the effect of different pretreatment method, enzymatic removal of starch, isoelectric precipitation of protein residue, and precipitation of water-soluble polysaccharides with ethanol solution on the yield and purity of β-glucan were systematic studied. From the single factor and orthogonal test, the optimum extracting conditions were fixed.The preparation produced 90% β-glucan.