摘要
建立了定量测定牛初乳IgG的免疫透射比浊法,确定该检测方法的最佳反应温度37℃,检测时间40min,浓度在0.07～0.75mg/ml范围内线性关系良好。该方法准确性好,回收率高,与火箭免疫电泳法比较,测定结果差异不显著,这说明免疫透射比浊法检测IgG含量具有良好的可靠性。
Immunoturbidimetry method was developed to determine the bovine colostrum IgG concentration. IgG was assayed by immunoturbidimetry with the reaction temperature 37℃ and reaction time 40 min, and with good linear relation ranged from 0.07 mg/ml to 0.75mg/ml. The immunoturbidimetry method showed good repeatability and stability in comparicon with the rocket immunity electrophoresis analysis, immunoturbidimetry method shows better reliability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第3期298-300,共3页
Food Science
基金
2007年农业行业标准制定项目
乳品科学教育部重点实验室主任基金项目(KLDS2006-0153)
