摘要
从测定漆酶活性的几种常用方法中,筛选出一种快速、稳定的灵芝漆酶测定方法:在基础培养基中加入0.04%愈创木酚,通过测定显色圈直径检测漆酶的活性;同时利用单因素分析和正交试验确定了灵芝的最适产漆酶条件:麦芽糖3%、酵母浸粉0.25%、pH值6.0、Cu2+0.5mmol/L,培养温度25℃。实验结果为灵芝的菌种选育及其漆酶的工业化生产提供了一定的参考。
A rapid and stable method for laccase assay in Ganoderma lucidum was screened from several different methods： the activity of laccase was detected by adding 0.04% guaiacol to the minimum medium and assaying the diameters of colored rings; Meanwhile, the optimal conditions for laccase production were obtained by the means of single factor analysis and orthogonal method： maltose 3%, yeast extract 0.25%, pH value 6.0, Cu^2＋ 0.5 retool/L,, culture temperature 25℃. These results can provide some references for the breeding of G.lucidum and industrialization of its laccase production.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第3期310-313,共4页
Food Science
关键词
灵芝
漆酶测定
单因素分析
正交试验
Ganoderma lucidum
laccase assay
single factor analysis
orthogonal test
