摘要
用丙二醛(MDA)试剂盒测定MDA的含量,用分光光度法测定大鼠红细胞溶血程度。结果显示:在体外,10.00mg/ml玉米多肽使自由基诱导的大鼠肝线粒体MDA生成抑制率达39.63%,对肝组织匀浆MDA生成抑制率分别为22.83%(正常组),45.59%(Fe2+诱导组)及67.53%(H2O2诱导组)。玉米多肽能抑制自由基诱导的大鼠肝线粒体肿胀,使大鼠肝线粒体肿胀度低于对照组,且剂量关系明显,表明其具有保护大鼠肝线粒体氧化损伤的作用。玉米多肽对H2O2诱导的大鼠红细胞氧化溶血有抑制作用,表明其对大鼠红细胞的氧化损伤具有保护作用。
The effects of corn peptides（CP） on hemolysis of rats red blood cell were observed in the spectrophotometric method, while the reagent kits were used to analyze the content of malondialdehyde （MDA）. In vitro, the inhibiting rate of 10.0mg/ml CP on radical-induced MDA formation in rat liver mitochondria was 39.63 %, while 22.83% in normal rats fiver, 45.59% incubated with Fe^2＋ and 67,53% incubated with H2O2. CP made the radical-induced swelling of rats liver mitochondria obviously be lower than the control, so the results showed that CP could inhibit oxidation damage of rats liver mitochondria. Cp could also inhibit hemolysis induced by H2O2 during warm-bathing of rats red blood cell, so this result showed activity the protection held against oxidation damage of rats red blood cell.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第3期463-465,共3页
Food Science
关键词
玉米多肽
抗氧化
体外
corn peptides （CP）
antioxidation
in vitro
