摘要
以松口蘑Tricholoma matsutake子实体为外类群,对大白口蘑T.giganteum野生子实体及其组织分离菌丝进行ITS序列测序,通过DNAStar软件进行比较分析。结果表明大白口蘑ITS序列长度为589bp,松口蘑ITS序列长度为601bp,ITS1和ITS2呈现不同程度的种间多态性;ITS序列测定证实了大白口蘑野生子实体及其组织分离菌丝的同质性,并且ITS区序列在大白口蘑种内不同菌株间的变异程度很小,表明使用通用引物ITS4和ITS5,通过PCR扩增测序即可用于大白口蘑的种质鉴定。
The total ITS rDNA sequences of fruitbodies of Tricholoma giganteum and Tricholoma matsutake, and the mycelia of cultures isolated from fruitbodies of T. giganteum were obtained and analyzed. The results indicated that the length of entire ITS of T. giganteum was 589bp, and T. matsutake 601bp. The length discrepancy and sequence polymorphism of ITS1 and ITS2 were existed between the two species, but there waslittle ITS variability of intraspecies in T. giganteum. The mycelia isolated had the same ITS sequences as their original basidioearps. The results also showed that the germplasm of T. giganteum could be diagnosed by ITS sequences.
出处
《菌物学报》
CAS
CSCD
北大核心
2008年第2期230-236,共7页
Mycosystema
基金
贵州省科技厅科学技术基金资助项目(No.20072043)
关键词
内转录间隔区
多态性
同质性
internal transcribed spacer （ ITS ）, polymorphism, homogeneity
