中国黑木耳主要栽培菌株ISSR指纹分析及SCAR标记

ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China

采用ISSR标记技术对来自全国的黑木耳34个主要栽培菌株进行DNA指纹分析,初步构建其标准化DNA指纹图谱;在ISSR指纹分析基础上进行聚类分析,并将黑木耳两个栽培菌株173和186中扩增获得的ISSR特异性DNA带转化为可以直接用于菌株快速鉴定的SCAR标记。研究表明,我国黑木耳栽培菌株遗传背景差异不大,存在同物异名现象,而采用ISSR指纹及其SCAR标记鉴定黑木耳栽培菌株具有重要意义。

Inter-simple sequence repeat（ISSR） was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region （SCAR） markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains ofA. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula ispracticable and significative.