基于RAPD、ISSR和AFLP对西瓜枯萎病菌遗传多样性的评价

Assessment of genetic diversity in Fusarium oxysporum f. sp. niveum by RAPD, ISSR and AFLP analysis

利用RAPD、ISSR和AFLP分子标记技术对50个西瓜枯萎病菌株进行了分析。结果表明,21个RAPD引物、21个ISSR引物和21对AFLP引物分别对供试菌株扩增出113、134和389条带,三种分子标记的遗传相似系数比较一致,均可揭示西瓜枯萎病菌的遗传变异特点。三种分子标记产生的聚类分析结果存在一定差异,其中RAPD类群与生理小种和地理来源之间均不存在明显关系;而AFLP和ISSR类群与生理小种之间存在一定相关性,与菌株的地理来源关系不明显。

Fifty isolates of Fusarium oxysporum f. sp. niveum were analyzed with random amplified polymorphic DNA （RAPD）, interal simple sequence repeat （ISSR） and amplified fragment length polymorphism （AFLP） techniques. The results showed that each 21 primers of RAPD, ISSR and AFLP generated 113, 134 and 389 polymorphic bands respectively. These three molecular markers had consistent genetic coefficient of similarity and revealed the genetic diversity of isolates in Fusarium oxysporum f. sp. niveum. The cluster results produced by the three techniques were different. For RAPD groups, the relation with both physiological races and geographic regions of isolates were all inconspicuous. The AFLP and ISSR groups were generally associated with the physiological races, whereas there was no obvious relationship with geographic regions of isolates.