ELISA和Realtime-PCR筛检献血员HBV感染结果分析

Analysis of ELISA and Realtime-PCR screening blood donators' HBV infection

目的比较ELISA与Realtime-PCR检测献血员HBV感染的差异,探索准确高效的献血员筛选方法,为提高临床血源质量服务。方法采集502例献血员血样,分离血清,分别采用ELISA与Realtime-PCR方法进行HBsAg和HBV DNA双盲检测,对两法检测结果不一致的献血员2个月后随访并抽血复检。结果502例献血员中,两法初检HBV感染的一致率为97.61%(490/502)(S=5.333 3,P=0.020 9)。2个月后对检测结果不一致的12例献血员复检,有7例2种方法检查均阳性。结论2种方法的初检结果有较好的一致性,均可用于HBV感染检测。Realtime-PCR的检测效果优于ELISA。

Objective In order to compare the difference between ELISA and Realtime-PCR for Hepatitis B virus detection, and explore better way for screening blood donators and serve clinic blood with super quality. Methods Serum samples were collected from 502 blood donators. Then HBsAg and HBV DNA were double-blindly detected by ELISA and Realtime-PCR. Donators with different results were followed up and collected blood samples to be detected again 2 months later. Results of all 502 samples for the first time of detection, a high concordance rate was observed （97.61%, 490/502, S=5. 333 3,P=0. 020 9）, but 12 subjects showed different results by these two methods and they were rechecked 2 months later, 7 of them were positive by both methods. Conclusion ELISA and Realtime-PCR possess good concordance in first screening for blood donators. Each of them can be used for HBV detection while Realtime- PCR is better.