柯萨奇B组病毒IgM抗体检测方法的建立及在产科中的应用

Determination of IgM antibody to Coxsackie B virus in serum and its application in obstetrics

应用间接免疫荧光法检测孕产妇血清中柯萨奇B组病毒IgM抗体（CBV－IgM），并与ELISA法作比较。两法结果无显著性差异（X2=0．5，P＞0．5）。1370名早、中、晚期孕产妇血清CBV－IgM抗体检出率均高于非孕组（P＜0．05）。

IgM antibodies against coxsackie B virus (CBV-IgM) in serum were detected with indirect immunofluo-rescent technique in comparison with enzyme linked immunosorbent assay(ELISA). Results showed no sig-nificant difference between two methods(p>0.5).But immunofluorescent technique could be used in typing of coxsackie virus. The CBV-IgM in serum was detected in 1370 cases of early,middle and late pregnant women with this method. The infective rates were 8.53%, 7.59% and 7.71% respectively and there was significant difference in comparison with which of unpregnant women (3.33%). Most of related viruses were type of B3 and B5. All 100 serum in cord blood of newborn were CBV-IgM negtive. Since the coxsackie viruses could spread from mothers to infants through respiratory and digest tract,so the author considered that it is important to control the spread of coxsackie viruses by monitoring the CBV-IgM in pregnant wom-en.