大鼠死后心血吗啡浓度变化的HPLC检测

AN OBSERVATION OF MORPHINE CONCENTRATION CHANGES OF THE POSTMORTEM RAT’S CARDIAC BLOOD BY HPLC

采用高效液相邑谱分析技术（HPLC）检测治疗量及中毒量吗啡肌注大鼠死后心血中吗啡浓度变化。结果表明，以治疗量吗啡肌往大鼠，在死后96h内，心血中吗啡浓度随死后时间增加而显著升高（P＜0．01），吗啡浓度水平与死后时间里显著正相关；以中毒量吗啡肌注大鼠，在死后12h内，心血吗啡浓度无明显变化；死后24h、48h及96h，随死后时间延长，心血中吗啡浓度逐渐升高（P＞0．01），其递增强度不如治疗量吗啡组大鼠的明显.本研究证实，死后尸体心血吗啡浓度明显受生前剂量的影响，且在死后96h内，随死后时间增加．心血中吗啡浓度少数不断增高。

This study reports the observation of the rat's postmortem cardiac blood concehtration changes of morphine determined by the high performance liquid chromatography (HPLC) after intramuscular in jection of therapeutic and toxic dose morphine. The results showed,that the cardiac blood concentra tion of morphine increased significantly along with the prolongation of time elapsed 96h after death in the group of animals administered with therapeutic dose of morphine (P<0.01), The ratfs cardiac blood concentration of morphine did not change obviously within 12h after death,but increased gradu ally from 24h to 96h after death (Pr0.01 ) in the group amimals and ministered with toxic dose of morphine, however,the increase by legrees of the morphine was less than the therapeutic dose group. This study proves that the dose of morphine injected antemortem affects the postrnortem cardiac blood morphine levels.