中国人血清脱氧核糖核酸酶I(DNase I)的遗传多态性研究

GENETIC POLYMORPHISM OF HUMAN SERUM DNase I IN CHINESE POPULATIONS

应用薄层PAGIF（T=5％，C=3％）结合特异酶底物染色技术，调查了中国随机人群DNaseI遗传多态性的分布，检出在中国人群中两种常见的等位基因，即DNaseI*1和DNasel*2，其基因频率DNaseI*1为0．53，DNaseI*2为0．47。家系分析表明：子代个体谱带分别来自父亲和母亲，谱带在亲代和子代之间的传递符合孟德尔遗传规律。按Hardy－Weinberg法则进行吻合度检验，观察值与期望值一致。人血清DNaseI等电点经测定为4．0。

The results of DNase I phenotyping using PAGIF followed by zymograln detecting method ispresent and two commom alleles DNase I * 1 and DNase I * 2 were detected- The gene frequenciesare 0. 53 and .0. 47 respectively. The results of family analysis obey The Mendel Laws. The observednumbers of the phenotypes are in good agreement with the Hardy-weinberg equelibrium. The pI ispH4. 0.