摘要
目的研究环孢素A(CsA)对小鼠骨髓型正常巨噬细胞株Ana-1诱导Th1细胞转录因子T-bet的影响,探讨CsA在再生障碍性贫血(再障)治疗中的免疫抑制作用。方法①反转录聚合酶链反应(RT-PCR)方法检测Ana-1细胞株培养上清作用后正常小鼠外周血单个核细胞(PBMC)T-betmRNA的表达。②RT-PCR方法检测Ana-1细胞株培养上清加CsA作用后正常小鼠PBMCT-betmRNA的表达。结果①加入Ana-1细胞株培养上清后,T-betmRNA的表达水平为1.54±0.03,较对照组(1.03±0.06)增多(P<0.05);②加入Ana-1细胞株培养上清和CsA后,T-betmRNA的表达为0.66±0.02,较加入Ana-1细胞株培养上清组(1.54±0.03)减少(P<0.05)。结论CsA抑制巨噬细胞诱导T-bet的生成,可能是其在再障治疗中发挥免疫抑制作用的途径之一。
Objective To study the effect of cyclosporine A（CsA）on transcription factor T-bet of T helper 1 type ceils stimulated by mice bone marrow macrophage cell lines Ana-1 and evaluate their immunosuuppressive effects in treatment of aplastic anemia（AA）. Methods （1）stimulated by supematant of cultured mice bone marrow macrophage cell lines Ana-1 ,mRNA expression of T-bet in peripheral blood mononuclear cells （PBMC） in normal mice was measured by reverse transcription polymerase chain reaction （RT-PCR）. （2）stimulated by supematant of cultured Ana-1 cell lines and CsA,mRNA expression of T-bet was measured by RT-PCR. Results （1）stimulated by Ana-1 cell lines, the expression of T-bet in PBMC in normal mice was 1.54 ± 0.03, which was higher than that of normal controls （ 1.03 ± 0.06） （P〈0.05）. （2）the expression of T-bet was 0.66± 0.02 after supematant of cultured Ana-1 cell lines and 1 mg/L CsA being added, which was less than that only stimulated by supernatant of cultured Ana-1 cell lines （1.54±0.03） （P〈 0.05）. Conclusion It may be one of its immunosuppressive effects in treatment of AA that CsA restrains the production of T-bet.
