摘要
以烤烟K326、白肋烟TN86种子为材料,研究了不同烟草类型种子萌发期蛋白酶及相关物质变化动态。结果表明,蛋白酶活性、可溶性蛋白质、总游离氨基酸含量都随萌发进程呈上升趋势,其间虽各有所波动,但彼此的变化动态相似,并呈一定的相关性;峰值最高期蛋白酶活性在84h,可溶性蛋白质在96~168h,而总游离氨基酸在萌发结束时(336h),可溶性蛋白质滞后于蛋白酶活性的提高,而总游离氨基酸又滞后于可溶性蛋白质的提高;不同烟草类型种子萌发总体趋势相近,但起伏峰值有差异。
In order to investigate the change profiles of proteinase and its relative matters during seed germination, the flue-cured tobacco variety of K 326 and the burley tobacco variety of TN 86 were used in this paper. The results showed that there were positive correlations among the proteinase activity, the content of solu- ble protein, and the total soluble amino acid. These three indexes increased along with the seed germination. The peak of proteinase activity was found at 84 hours after treatment, however the same evidences for the content of soluble protein and the total soluble amino acid were found between 96 and 168 hours and at 336 hours after treatment, respectively. soluble protein second, and th During e profiles during seed germination value. seed germination, the proteinase activity changed first, the content of last was the total soluble amino acid. Three indexes showed the same change between flue-cured tobacco and hurley tobacco, but different in the peak
出处
《种子》
CSCD
北大核心
2008年第2期40-42,共3页
Seed
基金
云南省烟草公司"优质多抗烟草新品种选育及配套技术研究"项目(07A06)
关键词
烟草种子
萌发期
蛋白酶
可溶性蛋白质
总游离氨基酸
tobacco seed
germination stage
proteinase
soluble protein
total soluble amino acid