乙肝病毒前C区基因变异对干扰素疗效影响的研究

A Study of the Effect of Genetic Mutation of HBV pre Core on the Therapeutic Effect of IFN

以错配聚合酶链反应及限制性片段长度多态性分析，研究了ＨＢＶ前Ｃ区基因变异对干扰素疗效的影响。在２９例接受干扰素治疗的慢性乙型肝炎病人中，有前Ｃ区１８９６位Ｇ→Ａ突变者ＨＢＶＤＮＡ阴转７例（４６．６％，７／１５），追踪１年内全部复发；无变异者ＨＢＶＤＮＡ阴转７例（５０．０％，７／１４），追踪未见复发。表明ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变是影响干扰素远期疗效，导致停药后复发的重要原因之一。

Mismatched polymerase chain reaction and restriction fragment length polymorphism assay were performed to elucidate the influence of pre-core mutation of HBV genome on the therapeutic effect of IFN α-2b. In 29 patients treated with IFN α-2b, HBV DNA pre core mutation were detected in 15 patients(15/29 ,51.7%), HBV DNA of seven of these 15 patients ( 7/15 ,46.6%)turned to negative ,all of them were reactivated during one year follow up period. In the other 14 patients without pre core mutation, seven patients(7/14,50%)became serum HBVDNA negative, none of them were reactivated. It was showed that HBV DNA pre core mutation was one of the main factors effecting the response to interferon therapy.