脑缺血大鼠脑内Ⅱ型磷脂酶A_2 mRNA表达

The change of group Ⅱ phospholipase A 2 mRNA expression in ischemic brain in Wistar rat

采用改良Ｚｉｅｖｅ法及ＲＮＡ斑点杂交技术，分别检测了大脑中动脉阻塞后大鼠脑内磷脂酶Ａ２（ＰＬＡ２）活力及Ⅱ型ＰＬＡ２ｍＲＮＡ表达的变化，旨在了解脑缺血后脑内ＰＬＡ２活力的变化及参与脑缺血病理过程的ＰＬＡ２类型。研究发现：与假手术组相比，脑缺血组大鼠脑内ＰＬＡ２活力显著增高（Ｐ＜０．０５）；假手术对照组大鼠脑内Ⅱ型ＰＬＡ２ｍＲＮＡ表达水平很低，脑缺血组大鼠脑内Ⅱ型ＰＬＡ２ｍＲＮＡ表达明显增强。结果提示：Ⅱ型ＰＬＡ２参与了脑缺血病理过程，有必要进一步研究Ⅱ型ＰＬＡ２ｍＲＮＡ表达抑制剂对脑缺血的保护作用。

In order to study if,and which group of phospholipase A 2(PLA 2) is involved in brain ischemia,the improved Zieve method and RNA dot blotting technology were adopted to examine changes of PLA 2 activity and expression of group ⅡPLA 2 mRNA in ischemic brain in rat.The brain ischemia model was produced by occluding the left middle cerebral artery of rat for 3 hours with a varnish cylinder attached to a nylon thread inserted through the internal carotid artery.It was found that the PLA 2 activity in left forebrain in rat in brain ischemia group was increased markedly compared with that in sham control group (P<0.05).The expression of group Ⅱ PLA 2 mRNA was poorly detected in left forebrain in rat in sham control group,whereas group Ⅱ PLA 2 mRNA was abundantly expressed in brain ischemia group.These results guggest that group Ⅱ PLA 2 is involved in brain ischemia and it ,s necessary to study whether group Ⅱ PLA 2 mRNA expression inhibitor is useful in preventing ischemic brain damage.