阿维A酸对系统性硬皮病患者成纤维细胞增殖及分泌TGF-β_1的影响

Effect of acitretin on the proliferation and TGF-β_1 secretion of fibroblasts in systemic sclerosis

目的:确定阿维A酸对系统性硬皮病患者皮肤成纤维细胞增殖及分泌转化生长因子β1(TGF-β1)的影响。方法:原代培养系统性硬皮病(SSc)患者皮肤成纤维细胞(FB),使用不同浓度的阿维A酸作用48h,四甲基偶氮唑盐(MTT)方法及ELISA法测定阿维A酸对成纤维细胞增殖及分泌TGF-β1的影响。结果:阿维A酸可明显抑制患者皮肤成纤维细胞的增殖,显著减少成纤维细胞TGF-β1的分泌,并具有浓度依赖性。与空白对照组相比,差异有显著性(P<0.05)。结论:阿维A酸对SSc皮肤成纤维细胞的增殖及TGF-β1的分泌均有显著抑制作用,为其治疗硬皮病提供了理论基础。

Objective： To determine the effects of acitretin on the proliferation and transfer growth factor- β1 （TGF- β1 ） secretion of fibroblasts in systemic sclerosis （SSc）. Methods： SSc dermal fibroblasts were primarily cultured, and were treated with different concentrations of acitretin for 48 h. The cell proliferation was determined by MTT assay, and the level of TGF - β1 measured with ELISA. Results： Acitretin not only inhibited the proliferation of the cultured dermal fibroblasts of SSc, but also reduced the secretion of TGF -β1 in a dose - dependent manner. There was significant difference compared with blank control （P 〈 0.05）. Conclusion： Acitretin significantly inhibits the proliferation and TGF -β1 production in cultured SSc fibroblasts, which provides the base for the treatment of SSc.