3种抗结核药物致大鼠肝脏损伤血清ALT与肝脏病理相关性研究

Study on the correlation serum ALT and pathological changes of liver in rats

目的探讨主要抗结核药物致肝脏损伤血清丙氨酸氨基转移酶(ALT)与肝脏病理相关性研究。方法健康成年SD大鼠64只,随机分为8组。异烟肼(INH)组、利福平(RFP)组、吡嗪酰胺(PZA)组、IHH+RFP组、RFP+PZA组、INH+PZA组、INH+RFP+PZA组。对照组:生理盐水。分别灌药10d后处死大鼠,留取颈动脉血,制备血清测ALT,取肝脏标本,在显微镜、投射电镜下观察肝脏病理变化。结果在抗结核药物PZA单药组、多药组,随着ALT的增高,大鼠肝脏组织病理及电镜表现均出现不同程度病理性改变。结论单药组、两药组、三药组3种抗结核方案中ALT的增高差异有统计学意义(P<0.05或0.01),同时有病理性的肝细胞的特征性变化,单药组以PZA组改变明显。

Objective To investigate the correlation serum ALT and pathological changes of liver in rats. Methods 64 healthy adult SD tats were randomly divided into 8 groups, isonicotiny hydrazide（INH） group,Rifampicin （RFP） group,Pyrazinamied（PTA） group, INH ＋ RFP group, RFP ＋ PZA group, INH ＋ PZA group, INH ＋ RFP ＋ PZA group and control group（ NS）. After 10 days, the rats were killedand their serums were taken and ALT was detected, at the same time, the rats＇ livers were collected and the pathological changes of liver were observed under light microscope and projective electron microscope. Results In different drugs groups, with the increase of ALT, the different degrees of pathological changes of liver were found. Conclusion There is a significant difference in serum ALT in single drug groups, two drugs groups and three drugs groups, meanwhile, there is characteristic pathological changes of hepatic cells, the more drugs, the worse injury of liver. In single drug group, the changes in PZA group are most obvious.