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氯化物协同转运蛋白KCC2在正常大鼠内耳中的表达及意义 被引量：2

Significance and Expression of Cation-chloride Contransporter KCC2 in the Rat Cochlea
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摘要 目的研究氯化物协同转运蛋白KCC2(potassium-chloride cotransporter-2)在内耳中的表达及分布。方法用免疫荧光素FITC(异硫氰酸荧光素)标记的方法检测KCC2在正常大鼠内耳的分布,荧光素标记阳性部位揭示大鼠耳蜗内KCC2的分布情况。结果KCC2的阳性表达部位主要分布在耳蜗的盖膜、柯蒂器、螺旋神经节细胞以及前庭壶腹嵴顶部的毛细胞,耳蜗螺旋韧带及血管纹上KCC2为弱阳性表达。结论在正常大鼠的内耳中,KCC2在耳蜗和前庭中有表达,提示KCC2可能通过对K+、Cl-转运的控制,配合其它的离子通道共同维持淋巴液中K+、Cl-的离子平衡。 Objective To investigate the distribution of KCC2（potassium--chloride cotransporter- 2） in the cochlea of normal rat and to examine the significance of this distrbution. Methods The KCC2 in inner ear of rats was labeled with FITC and monitored using fluoresence microsope. Fluoresence intensity of KCC2 revealed the distribution of KCC2 in the rat cochlear. Results The results showed hyper fluorescence was located in tectorial membrane,spiral ganglion,organ of corti. There were less intensity of fluorescesce among the stria vascularis. Conclusion KCC2 was expressed in the cochlea and vestibule of rats. The presense of KCC2 in the inner ear, combined with other ion channels, may maitain the ionic equilibriam in the lymph fluid.
作者 章晓军 黄治物 杨琨 肖伯奎 黄娟
机构地区 武汉大学人民医院耳鼻咽喉一头颈外科
出处 《听力学及言语疾病杂志》 CAS CSCD 2008年第2期145-147,共3页 Journal of Audiology and Speech Pathology
关键词 KCC2 毛细胞 螺旋神经节 KCC2 Hair cell Spiral ganglion
分类号 R764.43 [医药卫生—耳鼻咽喉科]
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参考文献15

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共引文献4

同被引文献42

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听力学及言语疾病杂志
2008年 第2期

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