核小体结合蛋白1在雄激素非依赖性前列腺癌中的作用

Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma

目的明确核小体结合蛋白1（NSBP1）在雄激素非依赖性前列腺癌细胞（PC-3）中的生物学功能。方法通过RNA干扰技术，使PC-3细胞中的NSBP1表达水平明显降低，然后用胆囊收缩素（CCK）-8法观察细胞活性的变化，用流式细胞光度术检测细胞周期的变化。结果NSBP1的表达抑制后，从60h开始PC-3细胞活性（A值为0．329±0．017与0．386±0．022）有显著下降（P〈0．05），抑制率到108h达到高峰（27．5％）。流式细胞光度术检测结果显示，NSBP1抑制后的PC-3细胞的G2M＋S期细胞百分率也从60h开始（31．7％±2．0％）与对照组（37．3％±2．5％）有统计学意义（P〈0．05）。结论NSBP1在前列腺癌PC-3细胞中，对维持细胞活性及促进细胞生长增殖有重要作用。

Objective To investigate biological function of nucleosomal binding protein 1 （NSBP1） in androgen-independent prostate cancer cells. Methods Androgen-independent prostate cancer cells of the line PC-3 were cultured and transfected with recombinant inhibiting siRNA expression plasmid pSilencer 2. 1- U6 neo. Before transfection and 12, 36, 60, 84, 108 and 132 h after transfection RT-PCR and Western blotting was used to detect the inhibition effect. CCK-8 was used to detect the viability of the cells. The cell cycle was examined by flow cytometry. Results The cell viability was significantly reduced since 60 - 108 h after the transfection （ all P 〈 0.05 ）, the inhibition ratio reaching climax 108 hours after the transfection （P=0.028）. However, the inhibition ratio began to increase. FC showed that since 60 h after the transfection the proportion of PC-3 cells at the G2M ＋ S phase began to increased compared to that of the control group （all P 〈 0.05 ）. Conclusion NSBP1 has important effect on maintaining cell viability and enhancing growth and proliferation of androgen-independent prostate cancer cells.