高压氧对缺血再灌注小鼠脑组织中细胞因子TNF-α、IL-6mRNA表达及血脑屏障通透性的影响

Effects of hyperbaric oxygen on cytokines TNF-α and IL-6 mRNA in the brain tissues and blood brain barrier after cerebral ischemia-reperfusion in mice

目的探讨高压氧（hyperbaric oxygen，HBO）对缺血再灌注小鼠脑组织中细胞因子TNF-α、IL-6mRNA的表达及血脑屏障通透性的影响。方法复制清醒小鼠脑缺血再灌注模型，并于再灌注期间行0．25MPaHBO治疗5次，在处死动物前1h经尾静脉注射2％伊文思兰（Evans blue，EB），采用RT—PCR及分光光度法分别检测脑组织中细胞因子TNF-α、IL-6mRNA的表达及EB的含量。结果细胞因子TNF-α、IL-6mRNA的表达及EB的含量缺血再灌注组较假手术组高，且2组间差异有统计学意义（P〈0．01）；HBO组与假手术组相比差异无统计学意义（P〉0．05）；HBO＋脑缺血再灌注组与脑缺血再灌注组相比差异有统计学意义（P〈0．01）。结论HBO可从基因水平明显减少脑缺血再灌注细胞因子TNF-α、IL-6mRNA的表达，从而降低血脑屏障通透性，而对正常脑组织中TNF-α、IL-6mRNA表达的影响很小。

Objective To investigate effects of hyperbaric oxygen （HBO） on cytokines TNF-α and IL- 6 mRNA in the brain tissues and blood brain barrier （BBB） after cerebral ischemia-reperfusion in mice. Methods Using conscious mice cerebral ischemia-reperfusion models, 0.25 MPa HBO were applied 5 times during the reperfusion period, and 2% Evens blue （EB） was injected into tail veins before an hour when the animals were died. The expressions of TNF-α and IL-6 mRNA as well as the contents of EB were determined by RT-PCR and spectrophotometer. Results The expressions of TNF-α and IL-6 mRNA as well as the contents of EB successively increased in ischemia-reperfusion group compared with the sham surgery group （P 〈 0.01 ） , were similar to those of the sham surgery group （ P 〉 0. 05 ）, were successively decreased compared with ischemia-reperfusion group（ P 〈 0.01 ）. Conclusions HBO can significantly reduce the expressions of TNF-α and IL-6 mRNA and the permeability of BBB, but has little effect on normal cerebral tissues.